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KMID : 0380620110430020220
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Korean Journal of Food Science and Technology
2011 Volume.43 No. 2 p.220 ~ p.223
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Inhibitory Effects of Chrysanthemum zawadsaki Ethanolic Extract on FcεRI α Chain Expression
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Shim Sun-Yup
Byun Dae-Seok
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Abstract
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Human basophilic KU812F cells express a high affinity for IgE receptor, FcεRI, which plays an important role in immunoglobulin E (IgE)-mediated allergic reactions. Using the human basophilic KU812F cells, we assessed the inhibitory effects of the ethanolic extract from Chrysanthemum zawadsaki(CZ) on FcεRI α chain expression. Flow cytometric analysis showed that cell surface FcεRI α chain expression was suppressed when the cells were cultured with CZ extract. RT-PCR analysis showed that the mRNAs for FcεRI α chain decreased by the treatment of CZ extract. Moreover, CZ extract was also observed to result in a reduction in the release of histamine from anti-FcεRI antibody (CRA-1)-stimulated cells. These results suggest that CZ extract may exert anti-allergic activity through down-regulation of FcεRI α chain expression and a subsequent decrease in histamine release.
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KEYWORD
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Chrysanthemum zawadskii var. latilobum, FcεRI, histamine
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